c. jejuni 81-176  (MAST Group Ltd)

Journal: Foodborne Pathogens and Disease

Article Title: Anti- Campylobacter Probiotics: Latest Mechanistic Insights

doi: 10.1089/fpd.2022.0039

Figure Lengend Snippet: Anti- Campylobacter Probiotics in Poultry

Article Snippet: E. coli Nissle 1917 microencapsulation in alginate-chitosan nanoparticles , 2 × 10 8 CFU , Reduced C. jejuni 81–176 [ATCC ® BAA-2151TM] intracellular survival and invasion in HT-29 cells. , ↓IL-12A, IL-1B, IL-18, CXCL8, TNF, TLR-1, TLR-4, and TLR-6 , Mawad et al ( ) .

Techniques: Probiotics, Concentration Assay, In Vivo, Suspension, In Vitro, Translocation Assay, Functional Assay, Inhibition

Journal: Foodborne Pathogens and Disease

Article Title: Anti- Campylobacter Probiotics: Latest Mechanistic Insights

doi: 10.1089/fpd.2022.0039

Figure Lengend Snippet: Anti- Campylobacter Probiotics, Evidence at Preclinical Level

Article Snippet: E. coli Nissle 1917 microencapsulation in alginate-chitosan nanoparticles , 2 × 10 8 CFU , Reduced C. jejuni 81–176 [ATCC ® BAA-2151TM] intracellular survival and invasion in HT-29 cells. , ↓IL-12A, IL-1B, IL-18, CXCL8, TNF, TLR-1, TLR-4, and TLR-6 , Mawad et al ( ) .

Techniques: Probiotics, Concentration Assay, In Vitro, Activity Assay, Genetically Modified, Formulation, Suspension, In Vivo, Bacteria, Infection

Journal: Foodborne Pathogens and Disease

Article Title: Anti- Campylobacter Probiotics: Latest Mechanistic Insights

doi: 10.1089/fpd.2022.0039

Figure Lengend Snippet: In Vitro Evidence of Probiotics Efficient Against Campylobacter

Article Snippet: E. coli Nissle 1917 microencapsulation in alginate-chitosan nanoparticles , 2 × 10 8 CFU , Reduced C. jejuni 81–176 [ATCC ® BAA-2151TM] intracellular survival and invasion in HT-29 cells. , ↓IL-12A, IL-1B, IL-18, CXCL8, TNF, TLR-1, TLR-4, and TLR-6 , Mawad et al ( ) .

Techniques: In Vitro, Probiotics, Concentration Assay

Journal: Biomolecules

Article Title: Cj0683 Is a Competence Protein Essential for Efficient Initialization of DNA Uptake in Campylobacter jejuni

doi: 10.3390/biom13030514

Figure Lengend Snippet: Visualization of import of multiple DNA molecules within one DNA uptake location. Fluorescein (F) and Cy3 labelled DNA was either added for 30 min to separate C. jejuni 81–176 suspensions (( A ), n ≥ 5) or in parallel to one bacterial competent culture (( B , C ), n = 4) and the fraction of cells with F and/or Cy3 fluorescent foci were analyzed. In ( A ) the fraction of active cells relative to the total number of bacteria is shown, in ( B ) the fraction of cells with fluorescent foci relative to the overall fraction of competent cells is depicted. Green bars, cells with only fluorescein labelled DNA foci; orange bars, cells with only Cy3 labelled DNA foci; yellow bar, cells with both fluorescein and Cy3 DNA in one/or two single focus/foci; green/orange hatched bar, cells with at least two separate foci, with either fluorescein or Cy3 DNA. ( C ), overlay image of differential phase contrast (DIC), Cy3 and F channel. Green, fluorescein focus; orange, Cy3 focus.

Article Snippet: C. jejuni 81–176 [ ] from −80 °C stock cultures (MAST Group Ltd., Bootle, UK) was grown on Columbia blood agar plates supplemented with 5% defibrinated sheep blood (ColbA, Oxoid, Thermo Fisher Scientific Inc., Waltham, MA, USA) under microaerobic conditions (5% O 2 , 10% CO 2 , rest N 2 ; Binder, Tuttlingen, Germany).

Techniques: Bacteria

Journal: Biomolecules

Article Title: Cj0683 Is a Competence Protein Essential for Efficient Initialization of DNA Uptake in Campylobacter jejuni

doi: 10.3390/biom13030514

Figure Lengend Snippet: The fraction of competent C. jejuni and of DNA uptake locations per cell increased in time of contact with fluorescent DNA. Competent C. jejuni 81–176 were exposed to fluorescent DNA for different time periods and the fraction of cells with active DNA uptake was monitored in comparison to 10 min DNA uptake in H. pylori J99 ( A ). Proportion of cells with distinct number of DNA uptake locations ( B ). In ( B ), fraction of cells relative to total amount of competent cells are depicted. Mean and standard deviations were derived from five independent experiments.

Article Snippet: C. jejuni 81–176 [ ] from −80 °C stock cultures (MAST Group Ltd., Bootle, UK) was grown on Columbia blood agar plates supplemented with 5% defibrinated sheep blood (ColbA, Oxoid, Thermo Fisher Scientific Inc., Waltham, MA, USA) under microaerobic conditions (5% O 2 , 10% CO 2 , rest N 2 ; Binder, Tuttlingen, Germany).

Techniques: Comparison, Derivative Assay

Journal: Biomolecules

Article Title: Cj0683 Is a Competence Protein Essential for Efficient Initialization of DNA Uptake in Campylobacter jejuni

doi: 10.3390/biom13030514

Figure Lengend Snippet: The median amount of DNA within single foci of C. jejuni was constant over time and ~4-fold less than in H. pylori . C. jejuni 81–176 and H. pylori J99 were incubated with the same batch of fluorescent genomic C. jejuni DNA labelled with Cy3. Uptake was followed in time for C. jejuni and compared to 10 min uptake in H. pylori . ( A ) Analysis of DNA foci in C. jejuni (red; light to dark corresponds to incubation times) and in H. pylori (blue), sorted according to fluorescence intensity. ( B ) ( C. jejuni ) and ( C ) ( H. pylori ) distribution of fluorescence intensities as boxplots; the boxplot length corresponds to the interquartile range (IQR; 50%) of data, the horizontal bar indicates the median value; whiskers represent 1.5 × IQR or the maximum/minimum value of the dataset; dots, outliers. Upper panel, merged DIC and Cy3-channel example images of C. jejuni and H. pylori cells after import of DNA. Note that Cy3 exposure times were 25 ms for H. pylori and 100 ms for C. jejuni . Scale bar of 2 µm. One representative experiment (out of three) is shown.

Article Snippet: C. jejuni 81–176 [ ] from −80 °C stock cultures (MAST Group Ltd., Bootle, UK) was grown on Columbia blood agar plates supplemented with 5% defibrinated sheep blood (ColbA, Oxoid, Thermo Fisher Scientific Inc., Waltham, MA, USA) under microaerobic conditions (5% O 2 , 10% CO 2 , rest N 2 ; Binder, Tuttlingen, Germany).

Techniques: Incubation, Fluorescence

Journal: Biomolecules

Article Title: Cj0683 Is a Competence Protein Essential for Efficient Initialization of DNA Uptake in Campylobacter jejuni

doi: 10.3390/biom13030514

Figure Lengend Snippet: Biofilm formation is independent of active DNA transport or natural transformation in C. jejuni . Upon growth in microtiter plates for 72 h and suitable washing of unbound bacteria, crystal violet staining indicated the intensity of biofilm formation, measured as absorbance at 595 nm. C. jejuni 81–176 (wt) and its isogenic mutants Δ pilQ , Δ comE , Δ comEC and the respective complemented strains were tested, using Pseudomonas aeruginosa as reference for a typical biofilm forming bacterium. Horizontal bar, mean of ≥3 independent experiments; n.s., not significant; *, p < 0.05; ***, p < 0.001.

Article Snippet: C. jejuni 81–176 [ ] from −80 °C stock cultures (MAST Group Ltd., Bootle, UK) was grown on Columbia blood agar plates supplemented with 5% defibrinated sheep blood (ColbA, Oxoid, Thermo Fisher Scientific Inc., Waltham, MA, USA) under microaerobic conditions (5% O 2 , 10% CO 2 , rest N 2 ; Binder, Tuttlingen, Germany).

Techniques: Transformation Assay, Bacteria, Staining

Journal: Biomolecules

Article Title: Cj0683 Is a Competence Protein Essential for Efficient Initialization of DNA Uptake in Campylobacter jejuni

doi: 10.3390/biom13030514

Figure Lengend Snippet: Cj0683 plays a pivotal role for DNA uptake into the periplasm of C. jejuni . DNA uptake ( A ) and transformation rate ( B ) in 81–176 (wt) and the mutant strains Δ cj0683, Δ cj0683-compl and Δ cj0683 Δ comE . ( A ) Cells were either incubated with fluorescein- (green bars, except for Δ cj0683 Δ comE ) or Cy3-labelled (orange bars) genomic DNA of 81–176 for 30 min. ( B ) Transformation rate was determined using unlabeled genomic DNA containing streptomycin resistance. Error bars indicate standard deviation ( n ≥ 4).

Article Snippet: C. jejuni 81–176 [ ] from −80 °C stock cultures (MAST Group Ltd., Bootle, UK) was grown on Columbia blood agar plates supplemented with 5% defibrinated sheep blood (ColbA, Oxoid, Thermo Fisher Scientific Inc., Waltham, MA, USA) under microaerobic conditions (5% O 2 , 10% CO 2 , rest N 2 ; Binder, Tuttlingen, Germany).

Techniques: Transformation Assay, Mutagenesis, Incubation, Standard Deviation